■ 基本信息
啟動子: | CMV |
復制子: | pUC |
終止子: | SV40 poly(A) signal,hGH poly(A) signal |
質粒分類: | 哺乳系列質粒；哺乳表達質粒；pCMV系列質粒 |
質粒大小: | 6299bp |
質粒標簽: | N-3×FLAG |
原核抗性: | Amp |
真核抗性: | G418 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 293T等哺乳細胞 |
培養(yǎng)條件: | 37℃，5%CO2 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物:
| CMV-F（CGCAAATGGGCGGTAGGCGTG） |
3'測序引物: | hGH-PA-R（CCAGCTTGGTTCCCAATAGA） |
■ 質粒屬性
質粒宿主: | 哺乳細胞 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | G418
|
熒光標記: |
|
■ 質粒簡介
p3×FLAG-CMV-10表達載體是用于在哺乳動物細胞中建立N-3×FLAG融合蛋白的瞬時或穩(wěn)定細胞內的pCMV5表達載體的升級版。該載體在多克隆區(qū)域上游編碼三個相鄰的FLAG表位（Asp-Tyr-Lys-Xaa-Xaa-Asp）。這導致使用FLAG抗體的檢測靈敏度增加。第三個FLAG表位包括腸激酶識別序列，允許從純化的融合蛋白切割3×FLAG肽。
The p3×FLAG-CMV-10 Expression Vector is a 6.3 kb derivative of pCMV5 used to establish transient or stable intracellular expression of N-terminal 3XFLAG fusion proteins in mammalian cells. The vector encodes three adjacent FLAG? epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen expressing host.
p3×FLAG-CMV-10 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigenexpressing
host, such as COS cells.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1697 p3×Flag-CMV-10哺乳表達質粒.txt
