■ 基本信息
啟動子: | gpdA |
復(fù)制子: | pUC |
終止子: | trpC terminator |
質(zhì)粒分類: | 絲狀真菌 |
質(zhì)粒大小: | 5518bp |
質(zhì)粒標(biāo)簽: | 無 |
原核抗性: | Amp |
篩選標(biāo)記: | Bar |
克隆菌株: | DH5α |
培養(yǎng)條件: | 37℃ |
表達(dá)宿主: | 絲狀真菌 |
誘導(dǎo)方式: | 無需誘導(dǎo) |
5'測序引物: | 根據(jù)全序列設(shè)計 |
3'測序引物: | 根據(jù)全序列設(shè)計 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 絲狀真菌 |
質(zhì)粒用途: | 蛋白表達(dá) |
片段類型: | 基因CDS
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標(biāo)記: |
|
■ 質(zhì)粒簡介
pBARGPE1是一個絲狀真菌-大腸桿菌穿梭表達(dá)載體，利用gpdA promoter可啟動外源目的基因的表達(dá)，轉(zhuǎn)化至絲狀真菌中可用草胺膦Bar篩選。The plasmid carries a polylinker with 5 or 6 unique restriction sites flanked on the right by a fungal promoter directing transcription into the polylinker and flanked on the left by the trpC terminator. The plasmid carry a bar selectable marker and a lox-NotI-lox site for insertion into lambda and for site-specific recombination between lox sites.
pBARGPE1質(zhì)粒的參考文獻(xiàn)：Infection cycle of maize stalk rot and ear rot caused by Fusarium verticillioides.
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請下載：
ZK1063pBARGPE1真菌表達(dá)質(zhì)粒.txt
質(zhì)粒只保證關(guān)鍵序列正確，不保證表達(dá)效果。
