■ 基本信息
別名: | pCDH-EF1α-MCS-T2A-Puro |
啟動子: | EF1α |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 病毒系列，慢病毒克隆載體 |
質粒大小: | 7087bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 無須誘導，瞬時表達 |
5'測序引物: | 根據(jù)序列設計引物 |
3'測序引物: | 根據(jù)序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Puro
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熒光標記: |
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■ 質粒簡介
Most cell types (split and non split cells); primary cell differentiation pCDH-EF1-MCS-T2A-Puro lentiviral expression vector of lentiviral vector is based on HIV for cDNA; cloning and expression; transfection efficiency of cells, to establish a stable cell line; single promoter driven by EF1a promoter and gene transcription and resistance genes for bicistron; between the two with a T2A peptide to shear on the translation level on the self, to ensure that the same genes and resistance gene translation level.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK781pCDH-EF1-MCS-T2A-Puro慢病毒表達質粒.txt
