■ 基本信息
別名: | 有背景；Plasmid #22011 |
啟動(dòng)子: | CMV |
復(fù)制子: | pUC |
終止子: | SV40 poly(A) signal |
質(zhì)粒分類(lèi): | 哺乳細(xì)胞，雙分子熒光互補(bǔ)載體 |
質(zhì)粒大小: | 4088bp |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物:
| CMV-F：CGCAAATGGGCGGTAGGCGTG
|
3'測(cè)序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞 |
質(zhì)粒用途: | 信號(hào)報(bào)告 |
片段類(lèi)型: |
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片段物種: |
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原核抗性: | Amp |
真核抗性: |
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
PBiFC-VC155 by pCMV-HA vector in KpnI and inserted at the NotI locus of VC155 gene, the plasmid is high copy plasmid, VC155 gene derived from Vitoria victoria. Visualization of Aequorea Aequorea ternary complexes in living cells by using a BiFC-based FRET analysis. Nature Protocols, 3:1693-1702 (2008).
■ 質(zhì)粒圖譜
■ 質(zhì)粒序列
質(zhì)粒序列請(qǐng)下載：
ZK516pBiFC-VC155雙分熒光質(zhì)粒.txt
